Abstract:
Enzymatic
d
ecolorization of Malachite Green (MG) dye
was studied
using crude enzyme from a
newly isolated Bacillus cereus str
ain wwcp1. 98
% decolorization efficien
cy was achieved within 24 hours using
an initial dye concentration of 1.0 x 10
-
5
M. Batch experimental results revealed that the decolorization process
was highly dependent on contact time, initial MG concentration, aqueous solution temperature and pH.
Biod
egradation of
MG
dye was
monitored spectrophotometrically
and metabolites confirmed by thin layer
chromatography (TLC). The comparison of TLC chromatograms before and af
ter decolorization confirmed that
crude
protease
enzyme ha
d
the
ability
to degrade MG d
y
e
.
The r
esults provide evidence that
the
crude enzyme
from Bacillus cereus strain wwcp1 is an effective and potential candidate for industrial wastewater treatmen