Abstract:
Two experimental models simulating the traditional storage conditions prevalent in Kenya, i.e. the open basket model and the modern wooden box model, were used to study the rate of dissipation and fate of malathion residues in maize grains and beans stored for periods of up to one year at ambient temperatures averaging 23°C. The grain samples were initially treated with 10·36 mg kg−1 of radiolabelled malathion dust prior to storage and portions analysed at regular intervals for malathion, malaoxon and the transformation products isomalathion, malathion α-monocarboxylic acid and malathion β-monocarboxylic acid using a combination of chromatographic, radioisotopic and mass-spectrometric techniques.
The findings showed a gradual penetration of malathion into the grains in amounts which were slightly higher in maize than in beans irrespective of the method of storage. After 51 weeks of storage, 34–60% of the initial residues persisted in all the grains. The total residual levels were slightly higher in beans than in maize irrespective of the storage methods though the persistence was a little higher in the wooden box than in the open basket. The rates of dissipation of the pesticide from the grains decreased with storage time and followed a biphasic pattern. Applying first-order reaction kinetics, the following half-lives were obtained: maize grains stored in open basket: 194 days; maize grains stored in closed wooden box: 261 days; beans stored in open basket: 259 days; beans stored in closed wooden box: 405 days. Beans stored in the wooden box had higher levels of bound residues than those sampled from the open basket. This trend was similar in maize grains although the concentrations were lower. The analysis of malathion metabolites confirmed the degradation trend of the residues.