Abstract:
In this study, a biosealant obtained from microbial carbonate precipitation (MCP) was evaluated as an alternative to an epoxy-coating system. A bacterium Sporosarcina pasteurii strain ATCC 11859, which metabolizes urea and precipitates calcite in a calcium-rich environment, was used in this study to generate the biosealant on a PCB-contaminated concrete surface. Concrete cylinders measuring 3 in (76.2 mm) by 6 in (152.4 mm) were made in accordance with ASTM C33 and C192 and used for this purpose. The PCB, urea, Ca2þ, and bacterial cell concentrations were set at 10 ppm, 666 mM, 250 mM, and about 2.1 108 cells mL 1, respectively. The results indicate that the biosealed surfaces reduced water permeability by 1e5 orders of magnitude, and had a high resistance to carbonation. Since the MCP biosealant is thermally stable under temperatures of up to 840 C, the high temperatures that normally exist in the surrounding equipment, which may contain PCB-based fluids, have no effect on the biosealed
surfaces. Consequently, there is greater potential to obtain a stronger, coherent, and durable surface by MCP. No measurable amount of PCBs was detected in the permeating water, indicating that the leaching water, if any, will have a minimum impact on the surrounding environment.
