Browsing by Author "Krämer, Petra M"

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    Optical Immunosensor and ELISA for the Analysis of Pyrethroids and DDT in Environmental Samples
    (American Chemical Society, 2007) Krämer, Petra M; Weber, Cristina M; Kremmer, Elisabeth; Räuber, Christina; Martens, Dieter; Forster, Stephan; Stanker, Larry H; Rauch, Peter; Shiundu, Paul M; Mulaa, Francis J
    An optical immunosensor (AQUA-OPTOSENSOR) and ELISA (enzyme-linked immunosorbent assay) for the analysis of pyrethroids and DDT in river water and/or sediment, are described. The optical immunosensor consists of a bench-top optical read-out-device and disposable single-use sensor chips. ELISA was carried out in the coating antigen format. As examples, phenothrin (pyrethroid) and p,p'-DDT were chosen. Herein we describe the overall strategy, the set-up and principle of the immunosensor platform, and show representative results for immunosensor and ELISA analysis. The immunosensor employs fluorophore (Oyster®-645)-labeled monoclonal antibodies (mouse mAb Py-1 and rat mAb DDT 7C12), and makes use of the evanescent field, thus operating without washing steps. ELISA in the coating antigen format uses a second antibody labeled with peroxidase. Both, phenothrin and p,p'-DDT can be analyzed with these immunochemical techniques in the low ppb levels. Advantages and drawbacks of both immunochemical platforms are discussed