Differential induction of proteins in Anopheles gambiae sensu stricto (Diptera: Culicidae) larvae in response to heavy metal selection

dc.contributor.authorMireji, Paul O
dc.contributor.authorKeating, Joseph
dc.contributor.authorKenya, Eucharia
dc.contributor.authorMbogo, Charles
dc.contributor.authorNyambaka, Hudson
dc.contributor.authorOsir, Ellie
dc.contributor.authorGithure, John
dc.contributor.authorBeier, John
dc.date.accessioned2015-05-28T14:49:56Z
dc.date.available2015-05-28T14:49:56Z
dc.date.issued2006
dc.description.abstractInvestigations were conducted to establish the magnitude and pattern of differential expression of proteins due to generational selection of third instar Anopheles gambiae s.s. Giles larvae by cadmium, copper and lead heavy metals, the three possible common urban pollutants. A susceptible strain of A. gambiae s.s. third instar larvae was separately placed under selection pressure with cadmium, copper and lead at LC30 and controls through five generations. First, third and fifth generation selection survivors were screened for differentially expressed proteins relative to non-exposed control by two-dimensional gel electrophoresis. Distribution patterns of the spots were analyzed by χ2 or Fishers' exact test and variations in expressions between and within generations by ANOVA. Most differentially expressed spots were acidic and of low molecular weight among all metals and generations. Type of heavy metal and generation were the main indicators of variations in differential expressions. Variation between generations was most significant among cadmium-selected populations of which the most number of spots were induced in the fifth generation. Most spots were induced in the copper-selected population in the third generation. The induced protein spots may be the products from respective genes that respond to heavy metals and counter their toxicity, thus building A. gambiae s.s. tolerance to these pollutants. The differential pattern and magnitude of expressed spots have potential application as molecular markers for assessment of anopheline adaptation status to heavy metals, and provide insight into the extent of environmental pollutionen_US
dc.identifier.citationInternational Journal of Tropical Insect Science / Volume 26 / Issue 04 / December 2006, pp 214-226en_US
dc.identifier.urihttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=960656&fileId=S1742758406658955
dc.identifier.urihttp://hdl.handle.net/123456789/1148
dc.language.isoenen_US
dc.titleDifferential induction of proteins in Anopheles gambiae sensu stricto (Diptera: Culicidae) larvae in response to heavy metal selectionen_US
dc.typeArticleen_US

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