Enzymatic Decolorization of Malachite Green Dye by a Newly Isolated Bacillus Cereus Strain wwcp1

Abstract

Enzymatic d ecolorization of Malachite Green (MG) dye was studied using crude enzyme from a newly isolated Bacillus cereus str ain wwcp1. 98 % decolorization efficien cy was achieved within 24 hours using an initial dye concentration of 1.0 x 10 - 5 M. Batch experimental results revealed that the decolorization process was highly dependent on contact time, initial MG concentration, aqueous solution temperature and pH. Biod egradation of MG dye was monitored spectrophotometrically and metabolites confirmed by thin layer chromatography (TLC). The comparison of TLC chromatograms before and af ter decolorization confirmed that crude protease enzyme ha d the ability to degrade MG d y e . The r esults provide evidence that the crude enzyme from Bacillus cereus strain wwcp1 is an effective and potential candidate for industrial wastewater treatmen